An apparatus for thin layer vertical polyacrylamide gel electrophoresis with discontinuous buffer system, and its practical applications
نویسندگان
چکیده
منابع مشابه
System for simplified discontinuous-gradient polyacrylamide-gel electrophoresis.
We describe a modified polyacrylamide gel electrophoresis method with which plasma proteins are excellently resolved in 4 h. We made a three-layer discontinuous gradient gel with concentrations of 80,60, and 45 g of polyacrylamide per liter of tris(hydroxymethyl)aminomethane/ethylenediaminetetraacetic acid/borate buffer, pH 9.2. Time and material needed in the preparation of the gel was greatly...
متن کاملUseful buffer and gel systems for polyacrylamide gel electrophoresis.
Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN) have been compiled and characterized. Usefulness and applicability of the systems for clinical investigations are discussed and illustrated by...
متن کاملDiscontinuous electrophoresis on polyacrylamide gel slab.
D ISCOT1XUOUS ELECT1IO1’HORESIS in polyacrylamide rods has been used for separation of serum proteins (1). In spite of satisfactory resolution in tile rod, the technic renhains cumbersome, and comparison of the closely migrating cOnlpoflents inaccurate. Akroyd (2) reported discontinuous electrophoresis On polyacrylamide gel slab, which alleviates many of the difficulties of the rod. We llave co...
متن کاملA convenient apparatus for vertical gel electrophoresis.
‘T1HE INTRODUC”ION of starch gel as an electrophoresis medimn by Smithies k4) arkecl a significant advance in the analysis of serum proteins. The increased resolving power afforded by this medium is indicated by the fact that as many as 30 distinct components can be electrophoretically separated; only 5 or 6 are usually observed on paper or in free-solution electrophoresis. Acrylamide gel (2, 3...
متن کاملThin-layer acrylamide gel electrophoresis.
A thin-layer acrylamide gel electrophoresis technique suitable for analysis of serum or urine proteins is described in detail. The method gives sharp differentiation of protein fractions and is particularly suitable for the analysis of biological solutions containing proteins in low concentration.
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1974
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.19.143